Assessment of neuronal antibodies levels and cytokine profile in healthcare professionals

A review of research in the field of occupational medicine shows that one of the central places in it is occupied by a comprehensive diagnosis of an employee's health status, especially in situations of severe and prolonged stress, which often leads to complete or partial professional burnout. The profession of a medical professional occupies a key place among specialties with a high risk of occupational stress. The consequences of occupational stress are closely related to the threat of developing various diseases of the nervous, cardiovascular, immune and endocrine systems. There is a growing interest in the importance of antibodies to specialized structures of nervous tissue, which are considered as new-generation biomarkers in a number of neurological disorders. A significant number of studies show the role of the cytokine network in the pathogenesis of anxiety and depressive disorders caused by stress. The study aims to evaluate the serum concentrations of antibodies to regulatory proteins of the nervous tissue, pro- and anti-inflammatory cytokines and their interrelationships in the secondary medical staff to substantiate the early immunochemical indicators of changes in the nervous system. The authors conducted a laboratory immunological examination of the secondary medical staff of the therapeutic profile and the comparison group. Using enzyme immunoassay in blood serum, the authors determined IgG class antibodies to nervous tissue proteins and the content of proinflammatory cytokines (IL-1β, IL-6, IL-8, TNF-α, IL-4). In medical workers working under occupational stress, when compared with the comparison group, the authors revealed increased levels of AВ to nervous tissue proteins (S-100, Hol-R, GABA-R, DA-R, Ser-R), a change in cytokine balance characterized by hyperactivation of the proinflammatory immune response (IL-1β, IL-8) against the background of increased individual immunoreactivity in 75% of cases. Correlation analysis showed the presence of a relationship between AB to DA and IL-1β receptors. The results obtained indicate a more likely risk of developing neuroimmune inflammation in medical staff and allow us to identify the most informative biomarkers (AT to DA-R, IL-1β, IL-8) for diagnosing disorders in the nervous system. Limitations. The disadvantage of this work is the small number of employees. Ethics. The study was conducted in compliance with ethical standards in accordance with the Helsinki Declaration of the World Medical Association. The work was approved by the local Ethics committee of the East-Siberian Institute of Medical and Ecological Research (Protocol No. 5 dated 03/21/2023).