Eupalinolide B Inhibited the Proliferation and Migration of Ovarian Cancer Cells via Downregulating ST6GalI Mediated Sialylation

Research on ovarian cancer, the most dangerous female reproductive tumor, requires deeper understanding of its development and effective therapies. Eupalinolide B (EB), found in Eupatorium lindleyanum DC., shows potential against ovarian cancer, but its precise impact and mechanisms are unclear. This study aims to assess EB’s effect on ovarian cancer cells and uncover its underlying mechanism. The CCK8 assay was used to detect the effect of EB on the proliferation of ovarian cancer Anglne cells and A2780 cells. The wound healing assay was used to detect the effect of EB on the migration of Anglne cells and A2780 cell. The expression of ST6GalI was determined by WB. After 12, 24, and 48 hours of treatment, the proliferation activity and migration of EB-treated cells was significantly reduced. The inhibitory effect of EB on the proliferation and migration of ovarian cancer Anglne cells and A2780 cells showed a time-dependent and concentration dependent manner. WB results showed that after 48 h treatment with EB, the protein expression of ST6GalI in ovarian cancer Anglne cells and A2780 cells significantly decreased. EB inhibited the proliferation and migration of ovarian cancer Anglne cells and A2780 cells, and its mechanism of action may be related to the decrease in ST6GalI mediated sialylation levels.