Optimization of industrial cultivation of Actinobacillus pleuropneumoniae strains

Actinobacillus pleuropneumoniae is a gram-negative bacterium from the Pasteurellaceae family, being the causative agent of a severe respiratory disease - actinobacillosis pleuropneumonia, it has a significant impact on the health and productivity of pigs, and also causes serious economic damage to pig farms. Affected animals experience fibrinous-hemorrhagic, necrotic pleuropneumonia, often leading to death, chronic complications or carriage of bacteria . In the presented work, the selection of the optimal composition of the nutrient medium for deep cultivation of serotypes 2, 5 and 11 Actinobacillus was carried out. pleuropneumoniae for subsequent production of a vaccine against actinobacillosis pleuropneumonia in pigs. Cultivation was carried out on media with the appropriate parameters, and the accumulation of biomass was assessed based on optical concentration indicators measured by spectrophotometry. In addition, a parallel experiment was conducted to compare the accumulation of biomass in a nutrient medium for deep cultivation of Actinobacillus pleuropneumoniae of imported production to confirm the compliance of the domestic environment with all the necessary parameters for the growth of microorganisms, as well as to identify the possible advantages of the domestic environment over the imported one, for its subsequent use within the framework of import replacement.