Morphological and functional indicators of the organs of immunity and the digestive system in intestinal dysbacteriosis of lambs

The technology of keeping in limited areas, non-traditional feeding, significantly different from natural conditions, the presence of morphofunctional features of the organism of the early postnatal period contribute to the development of intestinal dysbacteriosis in animals. A decrease in colonization resistance in intestinal dysfunction contributes to the overgrowth of microorganisms in the biotopes of the digestive tract, including by increasing the number and spectrum of potentially pathogenic and pathogenic enterobacteria that form biofilms in chronic enteritis of animals. To study the morphological and functional parameters before the experiments, calves of the Holstein breed (n=10), lambs of the Aginskaya breed (n=10) of the neonatal period up to 7 days were divided into groups according to the principle of analogues: I – animals with signs of diseases of the digestive system; II – clinically healthy animals. Immunological parameters were determined using automatic analyzers. Morphometric studies were carried out, taking into account the length, cm; width, cm; thickness, mm; diameter, mm using a caliper, a curvimeter and a ruler, with a division value of 1,0 mm. For histological studies, the material was fixed in 10.0 % neutral formalin solution, the sections were stained for general purposes with hematoxylin and eosin. Dynamics of morphofunctional indices of immunity organs and digestive system in lambs intestinal dysbiosis was characterized by an increase in the total number of leukocytes, the level of T-killers and NK-cells, a decrease in the total number of T-lymphocytes, T-helpers, B-lymphocytes of blood; indicators of phagocytic index, phagocytic activity of neutrophils, NST-spontaneous and NST-stimulated, stimulation index. Signs of catarrhal and hemorrhagic gastroenteritis, bauhinitis, cholangiohepatitis, spleen hyperplasia, lymphadenitis of regional lymph nodes were observed. The etiologic structure of lamb digestive diseases caused by pathogenic enterobacterias is represented by E. coli, antigenic structure: О86:А20 (6.73 %); О26:А20 (4.81 %); О86:F41 (4.81 %); О20:К99 (2.88 %); О9:А20 (0.96 %); О26:F41 (0.96 %); О78:К99 (0.96 %); О119:А20 (0.96 %); K. pneumoniae (16.35 %); P. mirabilis (9.62 %); E. aerogenes (3.85 %).