Quantification of glucagon and oxyntomodulin by protein precipitation-immunoaffinity enrichment-LC-MS/MS
We have validated a method with a highly detailed standard operating procedure. We have characterized calibration materials to help maintain accuracy and achieve between-day and between-laboratory harmonization. The assay will be beneficial in better understanding α-cell health and glycemic control in diabetes and other diseases.
Authors
- Katrina Forrest
- Jessica O. Becker
- Andrew N. Hoofnagle (ORCID: https://orcid.org/0000-0002-6449-0243)
- Michael J. MacCoss (ORCID: https://orcid.org/0000-0003-1853-0256)
- Michelle A. Emrick (ORCID: https://orcid.org/0000-0001-6943-4446)
- Elisha Goonatilleke
- Huu‐Hien Huynh (ORCID: https://orcid.org/0000-0002-7814-3189)
- Sara K Shijo
Institutions
- Human Genome Sciences (United States) (US)
- University of Washington (US)
- University of Washington Applied Physics Laboratory (US)
Publication Details
- Journal
- Journal of Mass Spectrometry and Advances in the Clinical Lab
- Published
- 2025-04-01
- DOI
- https://doi.org/10.1016/j.jmsacl.2025.04.002
- Citations
- 1
- Primary Topic
- Diabetes Treatment and Management
- Type
- article
- Field-Weighted Citation Impact
- 1.29
Funders
- National Institutes of Health
- Diabetes Research Center, University of Washington
- National Institute of Diabetes and Digestive and Kidney Diseases