A comprehensive suite for extracting neuron signals across multiple sessions in one-photon calcium imaging
We developed CaliAli, a comprehensive suite designed to extract neuronal signals from one-photon calcium imaging data collected across multiple sessions in free-moving conditions in mice. CaliAli incorporates information from blood vessels and neurons to correct inter-session misalignments, making it robust against non-rigid brain deformations even after substantial changes in the field of view across sessions. This also makes CaliAli robust against high neuron overlap and changes in active neuron population across sessions. CaliAli performs computationally efficient signal extraction from concatenated video sessions that enhances the detectability of weak calcium signals. Notably, CaliAli enhanced the spatial coding accuracy of extracted hippocampal CA1 neuron activity across sessions. An optogenetic tagging experiment showed that CaliAli enhanced neuronal trackability in the dentate gyrus across a time scale of weeks. Finally, dentate gyrus neurons tracked using CaliAli exhibited stable population activity for 99 days. Overall, CaliAli advances our capacity to understand the activity dynamics of neuronal ensembles over time, which is crucial for deciphering the complex neuronal substrates of natural animal behaviors.
Authors
- Masashi Yanagisawa (ORCID: https://orcid.org/0000-0002-7358-4022)
- Deependra Kumar (ORCID: https://orcid.org/0000-0003-1529-7567)
- Zhe Dong (ORCID: https://orcid.org/0000-0002-7366-8939)
- Yoan Chérasse (ORCID: https://orcid.org/0000-0002-6139-8193)
- Yuki Sugaya
- Masanori Sakaguchi (ORCID: https://orcid.org/0000-0002-7211-9452)
- Pablo Vergara (ORCID: https://orcid.org/0000-0001-8601-9551)
- Iyo Koyanagi (ORCID: https://orcid.org/0000-0001-7566-1856)
- Yuteng Wang
- Yu Feng (ORCID: https://orcid.org/0000-0001-9068-3746)
- Masanobu Kano (ORCID: https://orcid.org/0000-0002-0725-3292)
- Denise J. Cai (ORCID: https://orcid.org/0000-0002-7729-0523)
- Sakthivel Srinivasan (ORCID: https://orcid.org/0000-0002-9484-4962)
- Tristan Shuman (ORCID: https://orcid.org/0000-0003-2310-6142)
- Toshie Naoi
- Takeshi Sakurai (ORCID: https://orcid.org/0000-0003-4474-6253)
Institutions
- University of Tsukuba (JP)
- RIKEN Center for Advanced Intelligence Project (JP)
- The University of Tokyo (JP)
- Icahn School of Medicine at Mount Sinai (US)
- Tsukuba International University (JP)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-04-11
- DOI
- https://doi.org/10.1038/s41467-025-58817-z
- Citations
- 4
- Primary Topic
- Neural dynamics and brain function
- Type
- article
- Field-Weighted Citation Impact
- 5.72
Funders
- Takeda Science Foundation
- Uehara Memorial Foundation
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science London
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency