Genome-Wide Identification and Expression Analysis of m6A Methyltransferase Family in Przewalskia tangutica Maxim.

<i>N</i>⁶-methyladenosine (m⁶A) RNA modification plays important regulatory roles in plant development and adaptation to the environment. However, there has been no research regarding m⁶A RNA methyltransferases (MT-A70) in <i>Przewalskia tangutica</i> Maxim. Here, we performed a comprehensive analysis of the MT-A70 family in <i>Przewalskia tangutica</i> (<i>PtMTs</i>), including gene structures, phylogenetic relationships, conserved motifs, gene location, promoter analysis, GO enrichment analysis, and expression profiles. We identified seven <i>PtMT</i> genes. Phylogeny analysis indicated that the seven <i>PtMT</i> genes could be divided into three groups; two MTA genes, three MTB genes, and two MTC genes, and domains and motifs exhibited similar patterns within the same group. These <i>PtMT</i> genes were found to contain a large number of cis-acting elements associated with plant hormones, light response, and stress response, suggesting their widespread regulatory function. Furthermore, the expression profiling of different tissues was investigated using RNA-seq data, and the expression of seven genes was further validated by qPCR analysis. These results provided valuable information to further elucidate the function of m⁶A regulatory genes and their epigenetic regulatory mechanisms in <i>Przewalskia tangutica</i>.