Deciphering the interplay between NEAT1, miR-139-5p, miR-129-5p, TGF-β1, and collagen type I in pelvic organ prolapse

Objective: Pelvic organ prolapse (POP) is a benign gynecological disorder characterized by the descent of pelvic organs due to weakened support structures.While the molecular mechanisms behind POP are not fully understood, previous studies have indicated an association with altered levels of collagen type I and transforming growth factor-beta 1 (TGF-1).Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), which are essential regulators of gene expression, may provide valuable insights into the diagnosis, prognosis, and treatment of POP.This study aimed to investigate the protein levels of TGF-1 and collagen type I, as well as the expression levels of miR-129-5p, NEAT1, and miR-139-5p key regulators of TGF-1 and collagen type I among patients with POP.Materials and Methods: Thirty-four POP patients and thirty healthy controls were included in the study.The expression levels of miR-129-5p, NEAT1, and miR-139-5p were measured using quantitative polymerase chain reaction on RNA extracted from fascia tissues.TGF-1 and collagen type I protein levels were assessed via ELISA.Results: Compared to healthy controls, POP patients exhibited significantly higher levels of miR-129-5p (p=0.011) and TGF-1 (p=0.000). Conclusion:The findings suggest that miR-129-5p may play a crucial role in the pathophysiology of POP.Future studies should explore the role of lncRNAs in regulating miR-129-5p and their potential relationship with POP.