Lipid peroxidation and hepatic catalase in burn injury

The article describes the results of studying the intensity of redox processes in the liver of rats in burn injury to the skin. The study was carried out on non-linear male rats with an average weight of 220 grams. Age 4-6 months. Laboratory animals were divided into 7 groups of 10 individuals each. Group 1 (control) included intact animals that did not undergo burn exposure. 2–7 experimental groups were laboratory animals exposed to burns and removed from the experiment on the 2-, 4-, 7-, 10-, 14-, 21st day after the burn, respectively. In all experimental groups, contact burn injury was simulated in the depilated interscapular region of the back under ether narcotic conditions. By affecting the skin, the burn injury triggers a cascade of sequential reactions at the level of the whole organism. By affecting the skin, the burn injury triggers a cascade of sequential reactions at the level of the whole organism. In conditions of endotoxicosis developing during burns, the liver is central to the restoration of metabolism, detoxification and preservation of homeostasis. The intensity of oxidation-reduction processes in the liver was evaluated by determining the intensity of lipid peroxidation and the activity of the antioxidant protection enzyme catalase. The activity of free radical and antioxidant processes was judged by the percentage of malondialdehyde, as well as by the rates of spontaneous and ascorbate-dependent lipid peroxidation. In the course of the study, an increase in the processes of free radical oxidation in the homogenate of liver tissue was established, as evidenced by an increase in the concentration of TBC-active products of lipid peroxidation, most pronounced on day 2 of the post-burn process. Oxidative stress that occurred against the background of a burn injury of the skin led to a weakening of catalase activity in the liver at all follow-up periods, most reduced by 2 days. Burn-induced oxidative stress was accompanied by activation of free radical oxidation processes in the liver, as evidenced by an increase in the concentration of TBC-active products, and rate indicators of lipid peroxidation. With the intensification of lipid peroxidation processes, the possibilities of the body’s antioxidant defense mechanisms decreased.