VALIDATION OF THE METHOD FOR DETERMINING POLYCYCLIC AROMATIC HYDROCARBONS IN PALM KERNELS FROM THE TONPKI REGION

A rapid method for determining Benzo(a)Pyrene (B(a)P), Benzo(a)Anthracene (B(a)A), Benzo(b)Fluoranthene (B(b)F), Chrysene (CHR), Fluoranthene (FLA), Benzo(k)Fluoranthene (B(k)F), Dibenzo(a,h)Anthracene (D(a,h)A), Benzo(g,h,i)Perylene (B(g,h,i)P), and Indeno(1,2,3,c,d)Perylene (IdcP) in palm kernels using HPLC-UV has been validated.The validation procedure was conducted in accordance with the French standardization association method (NFV03-110, 1998), following the specific requirements of ISO/DIS/15753 applicable to High Performance Liquid Chromatography.The coefficient of determination values (R2) for the 9 studied PAHs ranges from 0.9915 to 0.9985, indicating linear calibration curves.Linearity testing demonstrates the normal distribution within the calibration range of 0 to 1000 g/L.Most of the detection and quantification limits are below 0.2 g/kg, confirming the high sensitivity of this PAH analysis method.The coefficients of variation obtained during repeatability tests (ranging from 0.89 for BaA to 2.28 for BkF) and reproducibility tests (ranging from 2.29 for BghiP to 4.88 for BaA) demonstrate the stability, reliability, and precision of the HPLC chromatograph.Therefore, the method for analyzing the 9 PAHs can be considered acceptable.