COMPARATIVE ANALYSIS OF NUTRIENT MEDIA FOR CULTIVATION OF BRUCELLA MELITENSIS REV-1 STRAIN DURING DEEP CULTIVATION IN A BIOREACTOR

Brucellosis of farm animals caused by Brucella species is one of the zoonotic diseases causing enormous annual economic damage to the Republic of Kazakhstan. The purpose of the study was to conduct a comparative analysis of nutrient media within submerged cultivation in bioreactor, with an assessment of growth factors and accumulation kinetics of the B.Melitensis REV-1 strain, to plot growth curves. According to the results obtained, nutrient media and their composition are one of the decisive factors influencing the kinetics of brucellosis accumulation. Depending on the type and composition of the nutrient medium, the B.Melitensis REV-1 strain will undergo the cultivation cycle differently. When using a standard nutrient medium (control medium), the peak accumulation is 3x106 cells/cm3, has a standard S-shaped growth curve. When using composition No.1, the peak accumulation is 4x106 cells/cm3, has a standard S-shaped growth curve with acceleration of the logarithmic phase. When using composition No.2, the peak accumulation is 5x106 cells/cm3, has a standard S-shaped growth curve with acceleration of the logarithmic phase, and deceleration of the stationary phase. When using composition No.3, the peak accumulation is 5x106 cells/cm3, has a standard S-shaped growth curve with acceleration of the logarithmic phase and deceleration of the stationary phase. The study used microbiological and biotechnological methods for analyzing the growth and kinetics of accumulation of Brucella. The research results can be used in mathematical modeling of Brucella growth when using submerged cultivation methods in bioreactors, which will increase the efficiency and improve the production of veterinary immunobiological drugs and diagnostic test systems.