ANALYSIS OF THE ACCUMULATION KINETICS OF THE TRYPANOSOMA EQUIPERDUM STRAIN IN LABORATORY SETTING

Equine trypanosomiasis is an infectious disease caused by parasites of the Trypanosoma genus, specifically Trypanosoma equiperdum and Trypanosoma evansi. The disease is specific to horses and other members of the equine family (donkeys, mules, zebras). Trypanosomiasis is transmitted through sexual intercourse, making it unique among other trypanosomiasis, which are usually transmitted through insect bites. During the studies conducted at room temperature (±22℃), the peak concentration of trypanosomes in mice weighing 17 grams or more was 110,000 trypanosomes/cm3. In mice weighing 16 grams, the peak concentration of trypanosomes was 90,000 trypanosomes/cm3. In mice weighing 15 grams, the peak concentration of trypanosomes was 46,000 trypanosomes/cm3. Additionally, when using a thermostat (±30℃), the peak concentration of trypanosomes in mice weighing 17 grams or more was 97,200 trypanosomes/cm3, but the mice died before 72 hours after infection. In mice weighing 16 grams, the peak concentration of trypanosomes was 120,000 trypanosomes/cm3. In mice weighing 15 grams, the peak concentration of trypanosomes was 90,000 trypanosomes/cm3. Based on the data obtained, a higher ambient temperature and high body weight allow for faster accumulation of parasitic mass, but exceeding these indicators leads to accelerated mortality, preventing the desired result from being obtained. Parasitological, biochemical and statistical research methods were used to analyze the kinetics of accumulation of the trypanosoma equiperdum strain. The research findings emphasize the importance of defining precise conditions for the successful cultivation of trypanosomes, which has practical significance for further studies on the biology of these parasites and the development of diagnostic methods and strategies to combat the diseases they cause.