The Pivotal Role of Heat Resistance Genes in Bali Cattle and Its Strategic Utilization in Adapting to Global Warming

Abstract One of the steps taken is to analyze and utilize the primary genes related to body temperature homeostasis, which directly impacts livestock resistance to heat. This study aimed to profile the HSP70 gene in Bali cattle of 12 Bali cattle from UPTD Sobangan, Gianyar Regency, Bali Province, and UPTD Kupang Regency, NTT using sequencing techniques to obtain specific SNPs and analyze their expression in different maintenance systems (intensive and extensive). The results of the long sequence analysis of the HSP 70 gene in the 1926 bp coding region (ATG to TAG) showed a 31 bp indel in the 5’UTR region and 6 SNPs in the coding region with a total of 5 SNPs (c.90 C/T, c.156A/C, c.279 C/T, c.1446 C/T, c.1569 A/G, c.1596 G/A) synonymous and one SNP c.1800 G/C non-synonymous which causes a change in amino acids from glutamic acid (E) to aspartic acid (D) with a total of 641 amino acids. The expression of the HSP70 gene in Bali cattle that were maintained in an intensive and extensive environment showed no significant difference. The study proves that Bali cattle can adapt to different maintenance system conditions.