The Effect of Adding Penicillin on The Quality of Pig Semen

Abstract One type of antibiotic that can be used in the semen dilution process is penicillin. The penicillin can inhibit the growth of gram-positive bacteria. This study aimed to determine the effect of penicillin addition in the pig semen dilution process on the quality of pig semen after 24 hours of dilution. The pig used in this study was a male pig over 2 years old. The method used was a Completely Randomized Design (CRD), consisting of 4 treatments with 4 replications, namely P0 without penicillin in the diluent and P1, P2 and P3 with 0.3, 0.6 and 0.9 gr/l penicillin in the diluent respectively. The extender used in this study was the non-antibiotic Beltsville Thawing Solution (BTS). The parameters observed were Motility, Viability, Sperm Abnormalities and pH. The data was analyzed using Analysis of Variance (ANOVA) and continued with HSD test when significant different was observed between the treatments. The results showed that the addition of penicillin was not significantly different (P>0.05) on pH and abnormalities, but was significantly different (P<0.05) on motility, and very significantly different (P<0.01) on viability of sperm. The addition of 0.3 g/l penicillin was the best level in the pig semen dilution process.