Rumen Fermentability Profiles and Digestibility of Combination of <i>Camellia sinensis, Hibiscus rosa sinensis</i> and <i>Syzygium aromaticum</i> as Feed Additive : In vitro Assay

Abstract A prior study shown that the best combination of hibiscus leaf 0.3 mg/ml (Supplement 1) and tea by-product 2 mg/ml might enhance VFA synthesis. This study aimed to evaluate the impact of a mixture of Syzygium aromaticum oil, Camellia sinensis leaf meal, and Hibiscus rosa-sinensis on the in vitro fermentability and digestibility of rumen. There were five replications and four treatments in the fully randomized block design experiment. Among the therapies were the following: As a control, A1 uses forage:concentrate (60:40% DM); A2 uses control plus Supplement 1; A3 uses control plus Supplement 1 plus 0.02 mg/ml S. aromaticum oil; and A4 uses control plus Supplement 1 plus 0.04 mg/ml S. aromaticum oil. The following variables were noted: pH value, total volatile fatty acid (VFA) generation, ammonia (NH 3 ) concentration, dry matter digestibility (DMD), organic matter digestibility (OMD), pH, total gas output, ammonia (NH 3 ) concentration, and total volatile fatty acid (VFA) generation. The results indicated that adding 0.04 mg/ml of S. aromaticum oil enhanced total VFA (p&lt;0.01), decreased DMD (P&lt;0.01), lowered OMD, and decreased total gas production (P&lt;0.1). There was no discernible difference between pH levels and NH 3 concentrations (P&gt;0.05). In summary, rumen fermentation characteristics may be stimulated by adding up to 0.04 mg/ml of S. aromaticum oil.