2D Disposable Textile Stochastic Sensors Based on Nanolayer of Diamond Like Carbon – Ag Modified with Cyclodextrins for Fast Diagnosis of Hepatitis C

Hepatitis C virus is a widespread persistent viral pathogen that has a global distribution, with a significant infection burden affecting more than 170 million individuals. Determination of the hepatitis C antigen at very low concentrations is essential for its early diagnosis, but also for the assessment of the quality of the transfusion blood. Therefore, two disposable textile sensors based on combined diamond like carbon—Ag nanolayer which was modified with α −, and ß - cyclodextrins were constructed, characterized, and validated for the assay of the hepatitis C virus antigen. The wide working concentration ranges allow the reliable assay of the antigen from 10 ag ml −1 to 1 μg ml −1 and with high sensitivities (6.54 × 10 10 and 4.60 × 10 9 s −1 g −1 ml when the sensors based on α −, and ß -cyclodextrins, respectively were used for the assay of HCV antigen).The proposed sensors are stable in time and their design is highly reliable. Recoveries higher than 98.00% with relative standard deviation lower than 1.00% were recorded for the HCV from whole blood samples. Student—t test made at 99.00% confidence level, and F test performed at 95.00% confidence level proved the high reliability and accuracy of the method proposed for the assay of HCV antigen.