Callus Formation from Encapsulated Explants of Plant Diplocyclos Palmatus (Shivlingi)

- Conservation of plants through different methods is being done from many years because many important plants are getting reduced due to several reasons. Diplocyclos palmatus (Shivlingi) is a medicinal herb. To conserve Shivlingi, encapsulation and callus induction technique was performed. Encapsulation technique was done by preparing beads of explants like shoot tip and nodal segments by using different concentration of Sodium alginate and calcium chloride. Combination of 4% sodium alginate, 100 mM calcium chloride showed best initiation. Callus induction from encapsulated explants was done by preparing MS medium containing different concentration of BAP + NAA and control medium.