Morpho-molecular approach (NGS plus digital PCR) in diagnosis of malignant biliary strictures
The implementation of dPCR allowed the identification of nearly 40% of positive cases in just one working day. In cases of negative dPCR, the NGS procedure can start on the same extracted nucleic acid used for dPCR, requiring more time, but reaching a 75% sensitivity. More studies are required to identify other more sensitive and specific dPCR targets, but even if our algorithm does not increase diagnostic accuracy, the possibility of avoiding FISH and reaching a diagnosis in a more time- and money-saving fashion might be an important step.
Authors
- Stefano Chillotti (ORCID: https://orcid.org/0000-0002-3870-0075)
- Lucía Miranda (ORCID: https://orcid.org/0009-0005-6986-6317)
- Sara Coluccelli (ORCID: https://orcid.org/0000-0002-1475-4044)
- Dario de Biase (ORCID: https://orcid.org/0000-0002-0609-8817)
- Thais Maloberti (ORCID: https://orcid.org/0000-0002-8306-4653)
- Francesco Vasuri (ORCID: https://orcid.org/0000-0002-1145-6025)
- E Albertini (ORCID: https://orcid.org/0009-0003-8123-7885)
- Giovanni Tallini (ORCID: https://orcid.org/0000-0003-0113-6682)
- Antonia D’Errico (ORCID: https://orcid.org/0000-0002-6120-7692)
Institutions
- Ospedale "Santa Maria delle Croci" di Ravenna (IT)
- Azienda USL di Bologna (IT)
- Istituti di Ricovero e Cura a Carattere Scientifico (IT)
- University of Bologna (IT)
Publication Details
- Journal
- Pathologica
- Published
- 2025-04-01
- DOI
- https://doi.org/10.32074/1591-951x-1117
- Citations
- 2
- Primary Topic
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Type
- article
- Field-Weighted Citation Impact
- 4.01
Funders
- Ministero della Salute