SYNTHESIS AND CHARACTERIZATION OF FIVE-MEMBERED HETEROCYCLIC COMPOUNDS OF TETRAZOLE DERIVATIVES AND THEIR BIOLOGICAL ACTIVITY

The aim of this study is to synthesize five-membered heterocyclic compounds containing four nitrogen atoms and one carbon atom in their structure. These compounds are known as tetrazoles, derived from 4-aryl-2-amino-1,3-thiazole (4-1), which were synthesized by reacting acetophenone or its derivatives (4-CH3, 3-NO2, 2-Cl) with thiourea in the presence of iodine as a catalyst to initiate the reaction. The amine was used to prepare Schiff bases (5-13) by reacting with acetophenone or its derivatives (4-NO2, 4-Cl, 4-CH3, 2-Cl, 3-NO2). The prepared Schiff bases (5-13) were then used to synthesize tetrazoles by reacting with sodium azide. Thin-layer chromatography (TLC) was employed to confirm the completion of the reaction and ensure the absence of unreacted materials. The newly synthesized compounds were characterized using physical methods, including melting point measurements and color determination, along with spectral techniques such as infrared spectroscopy (IR) and proton nuclear magnetic resonance (¹H-NMR), (13C-NMR) and their biological activity.