Antimicrobial effect of Stem cells from human exfoliated deciduous teeth metabolites combined with Epigallocatechin-3-gallate: As In vitro study

Background/purpose: According to The Global Burden of Disease Study in 2016, oral health problems, especially periodontal disease, become the 11th most common globally, including dental cavities, periodontal disease, and oral fungal infection. Epigallocatechin-3-gallate (EGCG), the composition of green tea, has excellent benefits and plays a vital role in health, especially in regulating oral pathogenic bacteria activity that causes oral inflammation. Stem cells of human exfoliated deciduous teeth (SHED) metabolites also have great benefits and play an essential role in the health sector, especially as an alternative to regenerative therapy with its many bioactive activities. This study aims to prove the antimicrobial power of the SHED metabolite combined with EGCG against Fusobacterium nucleatum (F. nucleatum), Lactobacillus acidophilus (L. acidophilus), and Candida albicans (C. albicans). Materials and methods: The six different passages of SHED were prepared in Dulbecco’s Modified Eagle medium and added with EGCG. After 48 hours of incubation, the Mueller-Hinton agar medium diffusion method measured the inhibition zone. The research data was analyzed statistically. Results: The SHED metabolite was antibacterially active combined with EGCG against L. acidophilus and F. nucleatum bacteria, but there was no antifungal activity against the fungus C. albicans. Conclusion: The SHED metabolite combined with EGCG had antibacterial properties against L. acidophilus and F. nucleatum bacteria but did not have antifungal properties against C. albicans fungi.