Improvement of Diagnostic Methods for Carriers of HH2 and JH1 Fertility Haplotypes in Holstein and Jersey Cows

This work aimed to improve the Holstein and Jersey cow HH2 and HJ1 fertility haplotype diagnostics, which indicate early embryonic mortality.Studies were conducted on 150 cows and 78 breeding bulls of the Holstein breed and 150 cows and 6 bulls of the Jersey breed.The Republic of Kazakhstan's cow population had 5.4 and 3.4% haplotypes associated with cow fertility, respectively.Due to its low cost, speed, and restrictions exclusion, the Tetra-primer ARMS-PCR reaction is recommended for detecting heterozygous carriers of the HH2 fertility haplotype.The proposed real-time polymerase chain reaction method accurately and quickly distinguishes homozygous healthy Jersey cattle and heterozygous carriers of the HJ1 haplotype of fertility.The DNA content in the reaction mixture should not be below 15ng/L for a reliable reaction.Periodically screening breeding stock and bulls for genetic anomalies can help control the spread of harmful mutations.