Crotepoxide from Kaempferia rotunda L. Inhibit Pancreatic lipase In vitro

Obesity prevention by targeting the inhibition of pancreatic lipase enzyme currently attracts some researchers, especially studies aiming to determine natural products' antiobesity activity. Their natural ingredients have a variety of chemical structures, which are expected to have potential as an antiobesity agent. Kaempferia rotunda is a plant that has the potential to inhibit the activity of the pancreatic lipase enzyme. This study aims to test the inhibition of the Porcine Pancreatic Lipase (PPL) enzyme by fractions and Crotepoxide isolated from Kaempferia rotunda Ethanolic Extract (KRE). Isolation was carried out using bioassay-guided isolation with an in vitro pancreatic lipase enzyme inhibition test used for the assay. P-Nitro Phenyl Butyrate (PNPB) functions as the synthetic substrate. KRE was separated by solid-liquid partition to obtain n-hexane (HF), ethyl acetate (EAF), ethanol (EF), and the insoluble residue fraction (RF). EAF was the most potent fraction (inhibition of 30.54±0.95%). Vacuum Liquid Chromatography further separated this fraction to produce four fractions (Ea1-Ea4), Ea3 had the highest inhibition (48.29±1.26%). Compound 1 (colorless needle) was isolated using the P-TLC method from fraction Ea.3, was identified as Crotepoxide. Crotepoxide was showing a percent inhibition of 42.80±0.49%, while Orlistat was 74.04±0.13%. Inhibition of the pancreatic lipase enzyme suggests that these compounds may exert antiobesity through another mechanism of antiobesity.