THÀNH PHẦN HÓA HỌC VÀ HOẠT TÍNH KHÁNG VI SINH VẬT CỦA TINH DẦU THÂN GIẢ LOÀI CONAMOMUM VIETNAMENSE

Conamomum vietnamense, a newly identified species of the Zingiberaceae family, was discovered in Lam Dong, Vietnam, in 2022. This study examined the chemical composition and antimicrobial activity of the essential oil extracted from its pseudo-stems. The essential oil was obtained via hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC/MS). Monoterpene hydrocarbons (59.55%) were identified as the predominant class of compounds in this essential oil, with α-limonene (32.73%) and 1,8-cineole (12.71%) as two main components. Additionally, the essential oil demonstrated antimicrobial activity, with inhibitory efficacy as follows: E. faecalis ≈ C. albicans (MIC = 32 µg/mL) > B. cereus ≈ S. enterica (MIC = 64 µg/mL) > S. aureus ≈ P. aeruginosa (MIC = 128 µg/mL) > E. coli (MIC = 256 µg/mL). A molecular docking study was conducted to investigate ligand-protein interactions involving the major constituents of the essential oil and key proteins from E. faecalis, S. aureus, B. cereus, E. coli, and C. albicans. The present study revealed that 1,8-cineole and α-pinene exhibited strong binding affinities to E. coli, whereas (6E)-nerolidol demonstrated a higher interaction with the 1IYL protein against C. albicans. Furthermore, α-limonene and α-phellandrene exhibited notable antimicrobial activities against both C. albicans and E. coli. This is the first report on the chemical composition of C. vietnamense pseudo-stem essential oil and its antimicrobial activity.