CHIẾT XUẤT, PHÂN LẬP VÀ ĐÁNH GIÁ TÁC DỤNG ỨC CHẾ α-GLUCOSIDASE IN VITRO VÀ IN SILICO CỦA GINSENOSID Rd TRONG SÂM VIỆT NAM

Ginsenoside Rd is one of the four marker saponins in the rhizome of Vietnamese ginseng (Panax vietnamensis Ha & Grushsv.) indexed in the Vietnamese Pharmacopoeia V along with ginsenoside Rg1, ginsenoside Rb1, and majonoside R2. In the drug development for type-2 diabetic treatment, enzyme α-glucosidase has become the promising molecular target of certain developed drugs such as acarbose and miglitol. In this regard, the present study to investigate ginsenoside Rd deals with the chemical contents (chromatographic analysis, isolation, and structural confirmation) and, especially, the additional findings on its α-glucosidase inhibitory effects in vitro and in silico. Ginsenoside Rd showed weak inhibitory effect on the activity of α-glucosidase in vitro with the the IC50 value of 388.24 µg/mL in comparison with the positive control acarbose with the IC50 value of 129.81 µg/mL. The interactions of ginsenoside Rd with α-glucosidase were simulated by molecular docking analysis in silico with the optimal binding affinity of -8.1 kcal/mol based on the interactive hydrogen binding.