TỐI ƯU HÓA QUY TRÌNH CHIẾT XUẤT ĐỂ BÀO CHẾ CAO ĐẶC TAN THỐNG PHONG DỰA TRÊN CHẤT ĐÁNH DẤU BERBERIN CLORID

The Tan thong phong remedy consisting of Cortex Phellodendri chinensis, Rhizoma Atractylodis, Radix Achyranthis bidentatae, Herba Siegesbeckiae, Rhizoma Anemarrhenae, Fructus Chaenomelis is used in traditional medicine to treat arthritis and gout. The Tan thong phong viscous extract was prepared by reflux extraction with the ethanol-water mixture in the laboratory scale. Berberine chloride (BBR) is the main active compound of Cortex Phellodendri. In this study, the extraction process for preparing the Tan thong phong viscous extract was optimized to maximize both the content and extraction yield of BBR using an D-optimal experimental design and artificial neural network methodology. The optimal parameters of the process were established with the ethanol content of 90 %, the solvent-material ratio of 8 ml per gram and the extraction time of 90 min for each of the 3 times. The content and extraction yield of BBR were 1.29 % and 73.16 %, respectively.