TÁC DỤNG KHÁNG VIÊM VÀ KÍCH THÍCH MIỄN DỊCH CỦA CAO CHIẾT VÀ TINH DẦU TỪ QUẢ CÂY NHÀU

The anti-inflammatory and immunostimulating effects of crude extract and essential oil extracted from Morinda citrifolia L. fruit (Noni) were investigated by using a carrageenan-induced mouse paw edema test and cyclophosphamide-induced immunosuppressive model in mice. Celecoxib (a selective cyclooxygenase-2 inhibitor) and zymosan (a phagocytic enhancer) were served as reference groups, respectively. The results of gas chromatography-mass spectrometry (GC-MS) analysis identified 31 components in Noni essential oil in which octanoic acid ethyl ester (53.03%); octanoic acid (18.26%); hexanoic acid (14.59%), and octanoic acid methyl ester (5.22%) were main components. The Noni essential oil at an oral dose of 0.15 mL/kg (repeated doses for 5 days) attenuated mouse paw inflammation reached 37.62% after 3h of carrageenan injection and 41.91% after 24h of carrageenan injection as the same as celecoxib (25 mg/kg). The Noni crude extract (1 g/kg) exerted weaker anti-inflammatory effect than essential oil with 33.88% and 24.08% reduction of mouse paw inflammation, respectively. The Noni crude extract (1 g/kg) and essential oil (0.15 mL/kg) also enhanced phagocytic activity in cyclophosphamide-treated mice which the effect of essential oil was more potent than zymosan (10 mg/kg). Moreover, the Noni crude extract and essential oil increased total white blood cells, and the percentage of lymphocytes, and decreased the percentage of monocytes. In conclusion, this is the first report on the anti-inflammatory and immunostimulating effects of Noni essential oil, suggesting further studies on the mechanism and application of Noni essential oil in the treatment of inflammatory diseases.