Development and evaluation of real-time recombinase polymerase amplification assay for fast identification of Rhizopus arrhizus
The developed RPA assay for R. arrhizus demonstrates high diagnostic sensitivity(96 %), specificity(95 %), and low detection limit(18.58 copies). While initial testing using stored unfixed tissue showed promise, comprehensive clinical validation studies are needed to establish the assay's diagnostic utility across diverse clinical settings and specimen types.
Authors
- Malati Tudu
- Minakshi Sahoo
- Saurav Sarkar (ORCID: https://orcid.org/0000-0001-9589-5115)
- Supriya Sahu
- Rumita Dey
- Pritika Gahlot
- Pradipta Parida
- Vinaykumar Hallur (ORCID: https://orcid.org/0000-0002-1001-7717)
- Mukund Sableb
- Ashutosh Lenka
- Subhasmita Bahinipati (ORCID: https://orcid.org/0000-0002-3744-5332)
Institutions
- All India Institute of Medical Sciences Bhubaneswar (IN)
- KIIT University (IN)
Publication Details
- Journal
- Indian Journal of Medical Microbiology
- Published
- 2025-04-09
- DOI
- https://doi.org/10.1016/j.ijmmb.2025.100845
- Citations
- 1
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- Department of Science and Technology, Ministry of Science and Technology, India
- Science and Engineering Research Board