Design, Synthesis and Characterization of Novel Coumarin Derivatives as Potential Human Epidermal Growth Receptor - 2 Inhibitors for their Anti-Breast Cancer Activity

Cancer encompasses various diseases marked by abnormal, uncontrolled cell growth that can invade healthy tissues, spread to other body parts, and remains a major global health concern. The present work involves the synthesis of novel coumarin derivatives and their preliminary in vitro biological evaluation as anticancer agents against MCF-7 breast cancer cell lines. The newly synthesized compounds were character on the basis of various spectral analytical techniques like IR and 1H-NMR. In vitro testing was conducted using the MTT assay protocol, with each derivative tested at four concentration levels: 10 µgmL-1, 20 µgmL-1, 40 µgmL-1, and 80 µg mL-1. The antiproliferative evaluation was done against breast cancer cell line. Compound 10 and Compound 1 were found the most potent compounds among the series against all the cell lines with IC50 values. Other molecules revealed good to moderate activity. This indicated that the molecules are safer for normal healthy cells.