Fingerprinting, Breast Cancer Inhibition of Premna serratifolia Extracts by In vitro and In silico Approaches

The side effects and drug resistance in breast cancer necessitate the search for new candidates from plant extracts, one of which is Premna serratifolia leaf. The leaf extract of P. serratifolia was tested for anti-breast cancer activity on MCF-7 cells and docked with the placental aromatase cytochrome P450 protein (PDB Code: 3S79). The most significant reduction of 50% in breast cancer cells was observed with the ethyl acetate extract at a concentration of 4.75μg/mL. Hydroxyl groups demonstrated the most substantial contribution to the anti-cancer activity with a correlation coefficient -3.167. Skrofulein, forming a complex with the protein 3S79, exhibited a binding energy of -5.69kcal/mol and an inhibition constant of 67.46µM. The study findings demonstrate that the ethyl acetate extract of P. serratifolia and NP-015559 have the highest potential to perform as agents against breast cancer.