MOLECULAR CHARACTERIZATION OF CUCUMBER MOSAIC VIRUS ASSOCIATED WITH CHILLI IN KOSHI REGION OF BIHAR INDIA

One of the most common vegetables and spices grown in tropical and subtropical regions of the world is the chilli (Capsicum annuum).Viral infections, which result in low fruit quality and quantity, pose a serious danger to chilli output.There are around 65 known viral diseases that may affect chillies worldwide.Cucumber mosaic virus is one of notorious virus; severely infecting the chilli worldwide; resulting into severe economic losses.In the present studies we have collected chilli samples showing mosaic disease from Koshi region (mainly Kasba, Jalalgarh and Dagarua) of Bihar during the period 2021-2022.Leaves and fruits with typical mosaic disease, chlorotic spots followed by mild mosaic and leaf distortion symptoms were observed under field conditions.Infected leaves were first screened by DAC-ELISA.Sap inoculation was carried on host plant from DAC-ELISA positive sample.Total RNAs were isolated from the host plant showing characteristic symptom of CMV after the post inoculation.Reverse transcription -polymerase chain reaction (RT-PCR) for coat protein (CP) gene of CMV with CP specific primers resulted into 657 bp amplification from the samples were collected from Kasba, Jalalgarh and Dagarua.