Studies on the Extraction of DNA from Human: Resourceful Material for the Identification

The extraction of DNA from human samples is a pivotal process in forensic science, medicine, and genetics, serving as a resourceful tool for identification purposes. This study explores various methods and techniques employed in DNA extraction from human tissues, focusing on their efficiency, reliability, and applicability in different contexts. Additionally, the impact of sample quality, degradation, and contamination on DNA yield and integrity is discussed. We compared 5 different methods to isolate DNA from human saliva and cigarette butts and result have been incorporated in form of gel images and UV-Spectrophotometer absorbance readings. Findings are represented in graphs for same sample with different methods.

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Publication Details

Journal
Journal of Applied Life Sciences International
Published
2025-04-09
DOI
https://doi.org/10.9734/jalsi/2025/v28i2684
Primary Topic
Forensic and Genetic Research
Type
article
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article

Studies on the Extraction of DNA from Human: Resourceful Material for the Identification

A. K. Alatafi, Meher Ali, Zulqurnain Ali, Zaheer Ahmed et al.
Journal of Applied Life Sciences International
Forensic and Genetic Research
article

Studies on the Extraction of DNA from Human: Resourceful Material for the Identification

A. K. Alatafi, Meher Ali, Zulqurnain Ali, Zaheer Ahmed, Noor Hassan Jumaa
article en

Abstract

The extraction of DNA from human samples is a pivotal process in forensic science, medicine, and genetics, serving as a resourceful tool for identification purposes. This study explores various methods and techniques employed in DNA extraction from human tissues, focusing on their efficiency, reliability, and applicability in different contexts. Additionally, the impact of sample quality, degradation, and contamination on DNA yield and integrity is discussed. We compared 5 different methods to isolate DNA from human saliva and cigarette butts and result have been incorporated in form of gel images and UV-Spectrophotometer absorbance readings. Findings are represented in graphs for same sample with different methods.

Journal of Applied Life Sciences InternationalVol. 28(2)
University of Diyala (IQ)
Openalex Percentile: Top 91%
Forensic and Genetic Research
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