Isolation and molecular identification of Parasediminibacterium sp. BT-541 isolated from soil

A Gram-negative, orange-colored bacteria, designated BT-541, was isolated from a soil sample in South Korea. Comparative analysis of 16S rRNA gene sequences revealed that the strain BT-541 exhibited the highest sequence similarity with Parasediminibacterium paludis HME6815T (98.50%) and 94.2-93.12% sequence similarity with other members of the family Chitinophagaceae. This strain could grow at temperatures ranging from 15 °C to 37 °C and pH ranging from 6-10. The only respiratory quinone in the bacterial isolate was menaquinone 7 (MK-7), and the major fatty acids were iso-C15:0, iso-C15:1G, and iso-C17:0 3-OH. Polar lipids included a phosphatidylethanolamine, three unidentified aminolipids, three unidentified aminophospholipids, an unidentified phospholipid, and five unidentified lipids. Molecular analysis and phenotypic characteristics indicated that strain BT-541 (KCTC 82123 = JCM 37057 = PQ 898028) could be a potential novel species in the genus Parasediminibacterium.