Long-term physical exercise facilitates putative glymphatic and meningeal lymphatic vessel flow in humans
Regular voluntary exercise has been shown to increase waste transport through the glymphatic system in mice. Here, we investigate the impact of physical exercise on both upstream and downstream brain waste clearance in healthy volunteers via noninvasive MR imaging. Putative glymphatic influx, evaluated using intravenous contrast-enhanced dynamic T1 mapping, increases significantly at the putamen after 12 weeks of long-term exercise using a cycle ergometer. The putative meningeal lymphatic vessel size and flow, measured by intravenous contrast-enhanced black-blood imaging and IR-ALADDIN technique, increase significantly after long-term exercise. Plasma proteomics reveals significant changes in inflammation-related and immune-related proteins (down-regulated: S100A8, S100A9, PSMA3, and DEFA1A3; up-regulated: J chain) after long-term exercise, which correlate with putative glymphatic influx or mLV flow. Our results suggest that increased glymphatic and mLV flow may be the potential mechanism underlying the neuroprotective effects of exercise on cognition, highlighting the importance of long-term, regular exercise.
Authors
- Sung‐Hong Park (ORCID: https://orcid.org/0000-0002-1731-0668)
- Roh‐Eul Yoo (ORCID: https://orcid.org/0000-0002-5625-5921)
- J. Y. Park (ORCID: https://orcid.org/0009-0001-9006-8820)
- Seung Hong Choi (ORCID: https://orcid.org/0000-0002-0412-2270)
- Hyo Youl Moon (ORCID: https://orcid.org/0000-0001-8207-1301)
- Jun-Hee Kim (ORCID: https://orcid.org/0000-0002-4080-0892)
- Yukyoum Kim (ORCID: https://orcid.org/0000-0002-4430-7659)
- Dohyun Han (ORCID: https://orcid.org/0000-0002-0841-1598)
- Hyun-Suk Shin
- Seongmin Cheon
Institutions
- Chonnam National University (KR)
- University of Seoul (KR)
- Seoul National University (KR)
- Korea Advanced Institute of Science and Technology (KR)
- Seoul National University of Education (KR)
- New Generation University College (ET)
- Seoul National University Hospital (KR)
- National University (SD)
- Institute for Basic Science (KR)
- National University College (PR)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-04-09
- DOI
- https://doi.org/10.1038/s41467-025-58726-1
- Citations
- 27
- Primary Topic
- Cerebrospinal fluid and hydrocephalus
- Type
- article
- Field-Weighted Citation Impact
- 24.55
Funders
- National Research Foundation
- Seoul National University
- Korea Basic Science Institute
- National Research Foundation of Korea
- Seoul National University Hospital
- Institute for Basic Science
- Samsung