Polyphenol briged bimetallic-heterostructure inducing chiral peroxidase nanozyme activity for enantiomers identification in gastric cancer
<sub>D</sub>-alanine (<sub>D</sub>-Ala) and <sub>D</sub>-proline(<sub>D</sub>-Pro) levels dramatically increase in the bio-fluids of gastric cancer (GC) patients, which are suggested to be potential biomarkers associated with GC. Therefore, monitoring the concentrations of <sub>D</sub>-Pro and <sub>D</sub>-Ala in the body fluids of GC patients is of great significance. In this study, a highly sensitive Surface Enhanced Raman Scattering (SERS) sensor has constructed based on bimetallic heterostructure nanoenzyme (3-MPBA: Co-TA@Ag NPs) for <sub>D</sub>-AAs detection in GC bio-fluids. Attributing to the formation of Ag-Co bimetallic dual active centers, the electron transfer behaviors accelerated boron hydroxyl group ionization in neutral pH, as well as the ultra-robust hydroxyl alcohol esterification reaction occurred between <sub>D</sub>-Ala and <sub>D</sub>-Pro and 3-MPBA. By simply testing different absorbance intensities at various concentrations and sample, a sensor for noninvasive detection of <sub>D</sub>-Pro/Ala is constructed with good specificity and efficiency. Besides, the quantitative determination of <sub>D</sub>-Pro/Ala (limits of detection (LOD) 2.37 μM and 2.15 μM for <sub>D</sub>-Pro/Ala. Furthermore, based on the differential level of <sub>D</sub>-Pro/Ala in GC bio-fluids, the differentiation of saliva samples with <sub>D</sub>-Pro/Ala can also be implemented by the proposed method, showing great potential in GC diagnosis.
Authors
- Zhiyong Guo (ORCID: https://orcid.org/0000-0003-1288-1573)
- Qiu-hong Yao
- Mei-huang Zeng
- Ting-xiu Ye
- Jingwen Jin (ORCID: https://orcid.org/0000-0001-9227-6837)
- Xi Chen (ORCID: https://orcid.org/0000-0002-3013-4558)
- Chen Zhang (ORCID: https://orcid.org/0000-0002-3579-6980)
- Xiaomei Chen (ORCID: https://orcid.org/0000-0003-1875-5869)
- Yi‐Ting Wang (ORCID: https://orcid.org/0000-0003-2008-4435)
- Feng Zheng
Institutions
- Jimei University (CN)
- Xiamen University (CN)
- Dalian Minzu University (CN)
- Xiamen University of Technology (CN)
Publication Details
- Journal
- Biosensors and Bioelectronics
- Published
- 2025-04-09
- DOI
- https://doi.org/10.1016/j.bios.2025.117449
- Citations
- 10
- Primary Topic
- Advanced Nanomaterials in Catalysis
- Type
- article
- Field-Weighted Citation Impact
- 5.18
Funders
- Natural Science Foundation of Xiamen City
- National Natural Science Foundation of China
- Natural Science Foundation of Fujian Province