Uptake, localization and dissolution of barium sulfate nanoparticles in human lung cells explored by the combination of ICP-MS, TEM and NanoSIMS
We show that BaSO<sub>4</sub> nanoparticles have been taken up by normal human bronchial epithelial cells (NHBEC) in a dose-dependent manner. However, no clear time-dependent uptake could be observed. The barium sulfate nanoparticles were visible in vesicles within the cytosol by TEM, which was confirmed by high-resolution NanoSIMS images. Nevertheless, no uptake of the particles into the nucleus was observed in this study. However, it was shown that BaSO<sub>4</sub>-NPs were partly dissolved, and barium ions were distributed throughout the entire cell. Toxicity of the particles was indicated by a dose- and time-dependent loss of viability in human lung cells.
Authors
- Martin Herzberg (ORCID: https://orcid.org/0000-0001-7746-0425)
- Dirk Dobritzsch (ORCID: https://orcid.org/0000-0002-0433-4345)
- Dirk Schaumlöffel (ORCID: https://orcid.org/0000-0003-3495-9677)
- Gerd Hause (ORCID: https://orcid.org/0000-0003-0242-3923)
- Sarah Thomas (ORCID: https://orcid.org/0000-0002-0053-0154)
- Maria Angels Subirana (ORCID: https://orcid.org/0000-0001-8723-7665)
- Felix Glahn
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Pau et des Pays de l'Adour (FR)
- Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (FR)
- Martin Luther University Halle-Wittenberg (DE)
Publication Details
- Journal
- Journal of Trace Elements in Medicine and Biology
- Published
- 2025-04-09
- DOI
- https://doi.org/10.1016/j.jtemb.2025.127650
- Citations
- 1
- Primary Topic
- Nanoparticles: synthesis and applications
- Type
- article
- Field-Weighted Citation Impact
- 0.52
Funders
- Deutscher Akademischer Austauschdienst
- Deutsche Forschungsgemeinschaft
- Agence Nationale de la Recherche
- Bundesministerium für Bildung und Forschung
- Campus France