Stable pleotropic loci controlling the accumulation of multiple nutritional elements in wheat

Understanding the genetic basis of nutrient accumulation in wheat is crucial for improving its nutritional content and addressing global food security challenges. Here, we identified stable pleiotropic loci controlling the accumulation of 13 nutritional elements in wheat across diverse environments using a large wheat population of 1470 individuals. Our analysis revealed significant variability in SNP-based heritability values across 13 essential elements. Genetic correlations among elements uncovered complex relations, with positive correlations observed within two distinct groups, where calcium (Ca), cobalt (Co), potassium (K), and sodium (Na) formed one group, and copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), molybdenum (Mo), nickel (Ni), phosphorus (P), and zinc (Zn) formed the other. Negative correlations were observed among elements across both groups. Through MetaGWAS analysis, we identified stable QTL associated with individual elements and elements with high positive correlations. We identified 67 stable QTL across environments that are independent from grain yield, of which 56 were detected using the MetaGWAS analysis indicating their pleiotropic effect on multiple elements. A major QTL on chromosome 7D that can shift the phenotype up to one standard deviation compared to the mean phenotype in the population exhibited differential effects on multiple elements belonging to both groups. Our findings offer novel insights into the genetic architecture of nutrient accumulation in wheat and have practical implications for breeding programmes aimed at enhancing multiple nutrients simultaneously. By targeting stable QTL, breeders can develop wheat varieties with improved nutritional profiles, contributing to global food security and human health.