Filtering cells with high mitochondrial content depletes viable metabolically altered malignant cell populations in cancer single-cell studies
This study provides insights into the functional characteristics of malignant cells with elevated pctMT, challenging current quality control practices in tumor single-cell RNA-seq analyses and offering potential improvements in data interpretation for future cancer studies.
Authors
- Josephine Yates (ORCID: https://orcid.org/0000-0001-8346-4502)
- Agnieszka Kraft (ORCID: https://orcid.org/0000-0001-7672-2032)
- Valentina Boeva (ORCID: https://orcid.org/0000-0002-4382-7185)
Institutions
- SIB Swiss Institute of Bioinformatics (CH)
- ETH Zurich (CH)
- Institute for Biomedical Engineering (CH)
- University Hospital of Zurich (CH)
Publication Details
- Journal
- Genome biology
- Published
- 2025-04-09
- DOI
- https://doi.org/10.1186/s13059-025-03559-w
- Citations
- 28
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 22.57
Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung