A METHOD FOR ANALYZING THE CONCENTRATION OF HEAT SHOCK PROTEIN 70 KDA IN SECTIONS OF BRAIN TISSUE OF TRANSGENIC MICE HSP70[IN] AND HSP70[EX] BY CLASSIFYING RAMAN SPECTRA

Introduction. Heat shock proteins (HSPs) are a group of highly prospective compounds that can be used in various branches of clinical medicine, such as neurology, pharmacology and oncology. Studying the content of these proteins in various parts of the brain of transgenic mice that express increased concentrations of exo- and endogenous HSP will make it possible to clarify the knowledge about the content of these proteins and their distribution in the structures of the central nervous system. The aim. To develop a method for identifying and analyzing the content of heat shock proteins weighing 70 kDa in sections of brain tissue of transgenic mice using the method of Raman spectroscopy. Material and methods. To conduct the experiment, were selected 9 mice of the C57BL/6 line (n=3 per group), among them the first group consisted of wild-type mice, the second and third were transgenic mice of the line with an integrated genetic cassette containing copies of the human heat shock protein expressed in the cytosol and into the interstitial space. In this study, Raman spectra of brain sections were measured using a Renishaw inVia Qontor confocal Raman microscope (Renishaw, UK). Raman spectra were recorded from the primary motor cortex and striatum of the brain with an accumulation time of 5 s, the laser excitation wavelength was 633 nm. Results. The obtained data underwent mathematical preprocessing, which included normalization, removal of outlines and baseline, dimensionality reduction, and noise filtering. There were trained 4 models for classifying Raman spectra, 2 of which showed high accuracy in differentiating spectra from brain sections of mice of the intact group and mice with overexpression of the 70 kDa heat shock protein. Conclusion. The obtained results demonstrate the feasibility of an attitude to data processing and interpretation, and also allow us to judge the prospects of using the method of Raman spectroscopy to analyze the distribution of heat shock proteins in brain tissue.