Optimization microwave-assisted extraction (MAE) to obtain total phenol from <em>Ampelocissus thyrsiflora</em> (Blume) Planch leaves for antibacterial activity response of <em>Staphylococcus Epidermidis</em>

Ampelocissus thyrsiflora (Blume) Planch. is a traditional North Sumatra medicinal plant that can be used to cure various ailments, such as wounds, diarrhea, infections, and as a stamina enhancer. A. thyrsiflora leaves contain secondary metabolite compounds such as alkaloids, flavonoids, tannins, and saponins, which have antibacterial properties. The extraction method used was microwave-assisted extraction (MAE) using ethanol solvent and different times and power extractions. The extraction process was optimized to obtain the optimum extraction conditions. Optimum extraction conditions can be developed as standardized herbal medicines. The method started with phytochemical screening and characterization of simplicia powder, extraction using MAE with 96% ethanol solvent with power variations of 180 watts, 300 watts, 450 W, and time variations of 3 min, 7 min, 15 min, determination of total phenol, and testing of antibacterial activity from optimization results using paper discs. The optimum extract yield was 20,20% at 450 watts of power and 15 min of time. Total phenol with optimum power and time of 232,0088±4,54 mg GAE/g sample was obtained from an ethanol extract of A. thyrsiflora leaves with 300 watts and 7 minutes is 6.25 mg/mL has an inhibition zone is 7,93±0,81 mm as bacterial minimum inhibitory concentration (MIC) and does not have minimum bacteria concentration (MBC) of Staphylococcus epidermidis bacteria. Ethanol extracts of A. thyrsiflora leaves with different extraction powers and times affected the yield of the extracts and total phenol. The antibacterial activity of the optimization result of ethanol extract of A. thyrsiflora leaves with the highest total phenol had MIC, but did not have MBC.