Comparison of the start-up of rotating biofilm contactor reactor with HN-AD bacteria inoculation under high and low influent ammonia conditions

Biofilm formation is critical for the engineering application of pure biofilm RBC processes inoculated with HN-AD bacteria. This study focused on comparing the startup of pure biofilm RBC systems inoculated with HN-AD bacteria under high ammonia nitrogen (NH₄⁺-N = 500 mg/L, H-RBC) and low ammonia nitrogen (NH₄⁺-N = 120 mg/L, L-RBC) influent conditions. The results showed that H-RBC shortened the biofilm formation time by 6 days. Additionally, the average removal efficiencies of ammonia nitrogen and TN were 30.70 % and 38.98 % higher than those of L-RBC, respectively. High-throughput sequencing indicated that compared with L-RBC, H-RBC did not significantly change the types of HN-AD bacteria but significantly increased the abundance of the key HN-AD bacterial genera Planktosalinus and Corynebacterium. Functional gene prediction analysis showed that the abundance of key functional genes affecting the nitrogen removal process, nirS and nosZ, in H-RBC was significantly higher than in L-RBC. Phenotypic prediction analysis revealed that H-RBC could better resist changes in the external environment and had stronger nitrogen removal capacity. These findings provide a theoretical basis and effective approach for the start-up of pure biofilm RBC system.