Effect of Phallus Impudicus Extract in Accelerating of Heard-Healing Wounds in Diabetes-Induced Rats

The multifunctional characteristics of Phallus impudicus extract encourage to conduct research for its potential use in medical applications. Diabetes mellitus is a metabolic disease, accompanied by chronic hyperglycemia caused by absolute or relative insulin deficiency. To purpose of this study was to show the potential role of Phallus impudicus extract in accelerating of wound healing in diabetes rats. For the induction of diabetes, streptozotocin (STZ, concentration 100 mg/ml) at a dose of 30 mg/kg b.w. in citrate buffer, pH 4.5 was used. Wounds were excised by cutting a 10-mm-diameter flap of full-thickness skin with a barb under inhalation anaesthesia, 5% isoflurane under aseptic conditions. Wounds were treating with standard used preparation and with prototype product containing an alcoholic extract of Phallus impudicus. A number of metabolites which promote wound healing including amino acids, sugars, organic acids and other compounds were identified in ethanolic extract by GC-MS. In the MTT test, the alcoholic extract of P. impudicus was non-toxic and stimulated fibroblast proliferation, which was also expressed as an increase in DNA and collagen synthesis. A hydrogel containing an extract of the fungus Phallus impudicus was shown to accelerate the period of wound desquamation in the experimental animals compared to the control groups. There was also a statistically significant (p>0.005) reduction in SOD activity and in TBARS compared to the control group, indicating an intensive healing process in which the oxidative enzyme systems play a significant role.