Anti-Inflammatory Activities of Lycopene on Xylene-Induced Ear Oedema in Mice and its In-Vitro Evaluation towards Protein Denaturation

This study aims to evaluate the anti-inflammatory activity of Lycopene in mice with ear oedema and in vitro activity. In this study, mice were used and performed in vitro anti-inflammatory tests of Lycopene with the HRBC method and protein denaturation. Moreover, the anti-inflammatory assay of Lycopene was also evaluated in xylene-induced right-ear mice. Diclofenac sodium solution was used as a positive control. The results showed that Lycopene provides anti-inflammatory activity in vitro by stabilizing the human red blood cell (HRBC), preventing lysis, and inhibiting protein denaturation with IC50 32.47 μg/ml. In addition, Lycopene also reduced the thickness of xylene-induced oedema in the right ear of mice. Lycopene provides anti-inflammatory properties by inhibiting HRBC lysis and protein denaturation and stabilizing HRBC, decreased the thickness of Oedema in the right ear of mice and the TNF-⍺ levels.