Soft 3D Bioelectrodes for Intraorganoid Signal Monitoring in Cardiac Models
Continuous monitoring of physiological activities within the internal regions of three-dimensional (3D) organoids holds significant promise for advancing organoid-based research. However, conventional methods are constrained to capturing signals from the peripheral surfaces of organoids, limiting insights into internal dynamics. Here, we present a soft 3D bioelectrode platform for continuous intraorganoid signal monitoring. These bioelectrodes, formed via 3D printing of liquid metal, are designed with customizable geometric parameters, including height and diameter, to adapt to various organoid structures. The tissue-comparable softness of the electrodes minimizes damage to cardiac organoids, ensuring a stable interface for reliable signal recording even under dynamic deformations caused by rhythmic contractions or displacements in aqueous environments. The array configuration enables simultaneous electrocardiogram (ECG) recordings from 32 organoids. Demonstrating real-time monitoring of drug-induced ECG responses, this scalable platform highlights its potential for high-throughput drug screening.
Authors
- Dayeon Kim
- Jang‐Ung Park (ORCID: https://orcid.org/0000-0003-1522-4958)
- Sumin Kim (ORCID: https://orcid.org/0000-0002-3117-7293)
- Suran Kim (ORCID: https://orcid.org/0000-0002-7981-5573)
- Seung‐Woo Cho (ORCID: https://orcid.org/0000-0001-8058-332X)
- Inhea Jeong (ORCID: https://orcid.org/0000-0003-2226-4755)
- Sungjin Min (ORCID: https://orcid.org/0000-0002-5041-8638)
- Yo Han Cho
- Enji Kim (ORCID: https://orcid.org/0000-0002-4934-8951)
- Jung Ah Lim (ORCID: https://orcid.org/0000-0002-3007-3855)
- Young‐Geun Park (ORCID: https://orcid.org/0000-0003-1579-0220)
- Sang‐Min Lee (ORCID: https://orcid.org/0000-0002-9089-5260)
- Hyebin Joo
Institutions
- University of Science and Technology (YE)
- Korea Advanced Institute of Science and Technology (KR)
- Yonsei University (KR)
- Center for NanoScience (DE)
- Institute for Basic Science (KR)
- Division of Materials Science and Engineering (AU)
- NanoBio (United States) (US)
- Convergence (US)
- Advanced Life Science Institute (JP)
- Korea Institute of Science and Technology (KR)
- Massachusetts Institute of Technology (US)
- Sungkyunkwan University (KR)
Publication Details
- Journal
- Nano Letters
- Published
- 2025-04-09
- DOI
- https://doi.org/10.1021/acs.nanolett.5c00069
- Citations
- 8
- Primary Topic
- Neuroscience and Neural Engineering
- Type
- article
- Field-Weighted Citation Impact
- 7.27