Time and Dose Dependent Effects of Triazine Derivatives on the Survivorship of Drosophila melanogaster

Triazine compounds are widely popular herbicides and recently, these have emerged as important therapeutic agents. Present investigation reports impact of two triazine compounds, 1,3,5-Triphenyl-[1,3,5] triazine (T1) and 1,3,5-Tris-(2-trifluoromethyl-phenyl)-[1,3,5] triazine (T2), on the survivorship of Drosophila melanogaster, a highly tractable and reproducible animal model for toxicological studies. We exposed adult flies to varying concentrations of T1 and T2 (125 mg/L, 250 mg/L, and 500 mg/L dissolved in DMSO) under standard culture conditions, comparing survivorship to control groups (untreated and DMSO-treated). Results indicated that both T1 and T2 do not show toxicity until day 30, however, post day 30 high dose of T1(500 mg/L) reduced survival percentage significantly. Derivative T2 was found to be non-toxic in the entire study period. One-way ANOVA and Tukey’s multiple comparison test were used to estimate the differences in mean survival percentage at specific time points using the software jamovi (v. 2.6.26). The findings highlight the differential effects of structurally related triazine compounds on D. melanogaster survival.