Coffea canephora var. Robusta Modulates Testosterone and CRISP-1 Levels in STZ-Induced Diabetic Rats

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia, oxidative stress, and inflammation, which can lead to various complications, including alterations in reproductive function. In this study, we aimed to investigate the effect of Coffea canephora var. Robusta ethanol extract on testosterone and cysteine-rich secretory protein-1 (CRISP-1) levels in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were divided into five groups: control, diabetic, and diabetic rats treated with Coffea canephora var. Robusta ethanol extract at three different doses (100, 200, and 400mg/kg body weight). After four weeks of treatment, serum testosterone and CRISP-1 levels were measured. The results showed that STZ-induced diabetes significantly decreased testosterone levels and increased CRISP-1 expression compared to the control group. However, treatment with Coffea canephora var. Robusta ethanol extract at all three doses significantly increased testosterone levels and decreased CRISP-1 expression in diabetic rats. Furthermore, the extract at the highest dose (400mg/kg) exhibited the most significant effect. These findings suggest that Coffea canephora var. Robusta ethanol extract may have therapeutic potential in regulating testosterone and CRISP-1 levels in STZ-induced diabetic rats, which could have implications for the management of diabetes-associated reproductive complications. Further studies are needed to elucidate the underlying mechanisms and evaluate the long-term effects of Coffea canephora var. Robusta ethanol extract on diabetes and its complications.