Extraction, amplification, and analysis of human DNA recovered from carrion insects of various postmortem intervals

In various different circumstances of crime scene recovery, it can be difficult to collect viable DNA samples of victims depending on the environment and condition of the remains. Forensic Entomology has played a crucial role in identifying rate of decomposition and time of death, however few studies have merged DNA analysis with this discipline making it a new route for identification. This research could aid in the identification of remains that are present and crime scenes with limited evidence. In order to produce results that can support these claims human DNA will be extracted from scuttle flies (Phoridae), carrion flies and their larvae (Sarcophaga bullata), and flesh eating beetles (Dermestidae) through the PrepFilerâ BTA Forensic DNA Extraction Kit, amplified through the use of human primers to isolate the SNPs (single nucleotide polymorphisms) and identify fractions of the human genome. The carrion insects will be subject to the intermittent feeding of chicken breast and human blood throughout the Instar stages of life that will determine which insect during the entirety of decay produces the most successful DNA analysis. The comparison of recovery and concentration of double stranded DNA via the NanoDrop and SNPs via the genetic analyzer will confirm the successful extraction of the DNA and its validation for application within the forensic community. This research was made possible by NASA West Virginia Space Grant Consortium, Training Grant #80NSSC20M0055.