Microwave-orchestrated phototherapeutic polypyrrole/soy extraction micro-complex for burn wound healing in a rodent model

Achieving effective burn wound healing while minimizing inflammation and preventing scarring remains a significant challenge in regenerative medicine. This research introduces a bioengineered composite that synergistically integrates near-infrared (NIR)-induced photothermal mild-hyperthermia with biotherapeutics to enhance skin lesion tissue regeneration . The composite leverages microwave-synthesized polypyrrole (PPy) for its efficient photothermal properties, combined with soy-derived Lactera (LAC), known for its anti-inflammatory and regenerative capabilities. Upon NIR exposure, the LAC-PPy microcomposite (MC) generates a mild hyperthermic environment that facilitates burn wound healing by promoting CD31-mediated angiogenesis , CD34-associated follicle activation, heat shock protein (HSP) expression, and modulation of oxidative stress , while regulating the anti-inflammatory M2 macrophage polarization . These mechanisms collectively establish a bioactive microenvironment that supports cellular proliferation and tissue remodeling. Immunofluorescence assays demonstrate notable upregulation of CD31, CD34, the M2 macrophage , and HSP within treated tissues, correlating with accelerated wound healing and improved skin structure restoration. The NIRtriggered photothermal response enables non-invasive phototherapeutic interventions, positioning the LAC-PPy MC as a promising strategy for managing burn injuries as well as complex wounds . By combining photothermal modulation with bioactive healing properties, the LAC-PPy MC represents an advancement in regenerative medicine, potentially improving clinical outcomes in burn wound care.