Phenogenomic resources immortalized in a panel of wild-derived strains of five species of house mice
The house mouse, Mus musculus, is a widely used animal model in biomedical research, with classical laboratory strains (CLS) being the most frequently employed. However, the limited genetic variability in CLS hinders their applicability in evolutionary studies. Wild-derived strains (WDS), on the other hand, provide a suitable resource for such investigations. This study quantifies genetic and phenotypic data of 101 WDS representing 5 species, 3 subspecies, and 8 natural Y consomic strains and compares them with CLS. Genetic variability was estimated using whole mtDNA sequences, the Prdm9 gene, and copy number variation at two sex chromosome-linked genes. WDS exhibit a large natural variation with up to 2173 polymorphic sites in mitogenomes, whereas CLS display 92 sites. Moreover, while CLS have two Prdm9 alleles, WDS harbour 46 different alleles. Although CLS resemble M. m. domesticus and M. m. musculus WDS, they differ from them in 10 and 14 out of 16 phenotypic traits, respectively. The results suggest that WDS can be a useful tool in evolutionary and biomedical studies with great potential for medical applications.
Authors
- Miloš Macholán (ORCID: https://orcid.org/0000-0001-5663-6831)
- Zuzana Hiadlovská (ORCID: https://orcid.org/0000-0003-4730-4557)
- Diethard Tautz (ORCID: https://orcid.org/0000-0002-0460-5344)
- Anna Bryjová (ORCID: https://orcid.org/0000-0003-4198-5486)
- Barbora Vošlajerová Bímová (ORCID: https://orcid.org/0000-0002-8658-5112)
- Jiřı́ Forejt (ORCID: https://orcid.org/0000-0002-2793-3623)
- Christine Pfeifle (ORCID: https://orcid.org/0009-0003-4762-0602)
- Jakub Kreisinger (ORCID: https://orcid.org/0000-0001-9375-9814)
- François Bonhomme (ORCID: https://orcid.org/0000-0002-8792-9239)
- Dagmar Čížková (ORCID: https://orcid.org/0000-0001-5031-7792)
- Jaroslav Piálek (ORCID: https://orcid.org/0000-0002-0829-7481)
- Ľudovít Ďureje (ORCID: https://orcid.org/0009-0000-9406-7377)
- Tatiana Aghová (ORCID: https://orcid.org/0000-0002-4184-4678)
- Iva Martincová (ORCID: https://orcid.org/0000-0001-5066-0849)
- Iva Pospíšilová
- Kateřina Janotová
- Jana Piálková
- Pavla Klusáčková
- Joë̈lle Goü̈y de Bellocq (ORCID: https://orcid.org/0000-0001-5831-6284)
- Helena Hejlová
- Ludmila Rousková
- Annie Orth
Institutions
- Centre National de la Recherche Scientifique (FR)
- École Pratique des Hautes Études (FR)
- Université de Montpellier (FR)
- Czech Academy of Sciences (CZ)
- Charles University (CZ)
- Masaryk University (CZ)
- Czech Academy of Sciences, Institute of Animal Physiology and Genetics (CZ)
- Institut des Sciences de l'Evolution de Montpellier (FR)
- Prague Zoo (CZ)
- Czech Academy of Sciences, Institute of Molecular Genetics (CZ)
- Czech Academy of Sciences, Institute of Vertebrate Biology (CZ)
- Max Planck Institute for Evolutionary Biology (DE)
- Institut de Recherche pour le Développement (FR)
Publication Details
- Journal
- Scientific Reports
- Published
- 2025-04-08
- DOI
- https://doi.org/10.1038/s41598-025-86505-x
- Citations
- 4
- Primary Topic
- Genetic Mapping and Diversity in Plants and Animals
- Type
- article
- Field-Weighted Citation Impact
- 7.27
Funders
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Grantová Agentura České Republiky
- Akademie Věd České Republiky
- Central European Institute of Technology
- Masarykova Univerzita