Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma
Here, we present a protocol for developing an in vitro 3D model of neuroblastoma through bioprinting, incorporating key components of the tumor microenvironment (TME), including vasculature and cancer cell line spheroids. We describe steps for the preparation, bioprinting, and evaluation of tumor models and their use to assess the impact of TME on solid tumor behavior. We report procedures for bioink preparation, bioprinting, the measurement of mechanical properties and fidelity, as well as the viability of different cell types and their interactions. For complete details on the use and execution of this protocol, please refer to Ning et al.<sup>1</sup>.
Authors
- Melissa Cadena (ORCID: https://orcid.org/0000-0003-1495-3681)
- Linqi Jin
- Liqun Ning (ORCID: https://orcid.org/0000-0002-3651-7410)
- Jenny Shim (ORCID: https://orcid.org/0000-0002-6717-2347)
- Kaveeta Kaw (ORCID: https://orcid.org/0000-0002-6456-8004)
- Mehdi Salar Amoli (ORCID: https://orcid.org/0000-0002-4292-1583)
- Martin L. Tomov (ORCID: https://orcid.org/0000-0002-3281-3220)
- Vikas S. Sridhar (ORCID: https://orcid.org/0000-0001-7947-1822)
- Morteza Mahmoudi (ORCID: https://orcid.org/0000-0002-2575-9684)
- Vahid Serpooshan (ORCID: https://orcid.org/0000-0002-5304-095X)
- Sarah Krikor
- Kelly C. Goldsmith (ORCID: https://orcid.org/0000-0001-8672-2298)
- Sarah Rezapourdamanab
- Mostafa Meselhe
Institutions
- Cleveland State University (US)
- Georgia Institute of Technology (US)
- Emory University (US)
- Children's Healthcare of Atlanta (US)
- Michigan State University (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-04-08
- DOI
- https://doi.org/10.1016/j.xpro.2025.103725
- Citations
- 3
- Primary Topic
- Neuroblastoma Research and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 4.17