An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation
Here, we introduce new Agrobacterium-mediated plant transformation vectors by introducing additional fluorophores to create the pJRA vector series. Additionally, we introduce the pLCS series of vectors, a new set of modular Gateway- and Gibson assembly-compatible vectors designed for protoplast transfection. All described vectors are available from Addgene to serve as a resource for the plant research community.
Authors
- Lucia C. Strader (ORCID: https://orcid.org/0000-0002-7600-7204)
- Sunita Pathak (ORCID: https://orcid.org/0000-0003-1351-760X)
- Michael Flanagan (ORCID: https://orcid.org/0000-0003-3925-1512)
- Ryan J. Emenecker (ORCID: https://orcid.org/0000-0001-7055-2773)
- Jeffrey R. Allen (ORCID: https://orcid.org/0000-0003-0148-3860)
- Samantha K Emenecker
Institutions
- Duke University (US)
- Washington University in St. Louis (US)
Publication Details
- Journal
- BMC Research Notes
- Published
- 2025-04-08
- DOI
- https://doi.org/10.1186/s13104-025-07231-1
- Citations
- 2
- Primary Topic
- Plant tissue culture and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Science Foundation
- National Institutes of Health